The present invention relates to methods of manufacture of immunogenic glycoconjugates,in particular for use in pharmaceutical compositions for inducing a therapeuticimmune response in a subject. The immunogenic glycoconjugates of the inventioncomprise one or more oligosaccharides or polysaccharides that are conjugatedto one or more carrier proteins via an active aldehyde group. Accordingly, theinvention provides methods of making (i) unsaturated microbial N-acyl derivativeoligosaccharides or polysaccharides； (ii) novel conjugates of unsaturatedN-acyl derivatives； and (iii) glycoconjugate compositions comprising conjugatemolecules of fragments of microbial unsaturated N-acyl derivatives that serveas a covalent linker to one or more proteins. The invention further encompassesthe use of the immunogenic glycoconjugates pharmaceutical compositions forthe prevention or treatment of an infectious disease.
