The present invention provides an plant activity composition for drug-free safety culture and its extraction method, in which medicinal materials, such as honeysuckle, golden cypress, andrographis paniculata, hawthorn, and licorice, are performed with extractions once respectively through solvents of water, ethanol, and ethyl acetate then, the extracts are concentrated and mixed to obtain the extract of each of the medicinal materials and these medicinal materials can be first mixed and then extracted or can be first extracted and then mixed to obtain the plant activity composition. In addition, it is applicable to use rhubarb, Astragalus membranaceus, or probiotics in the present invention. By using the bacteriostatic plant active ingredients and the effect of the combination ratio of the extracted plant activity composition having synergy, this invention is able to not only enhance the immunity for animals but also possess excellent feed conversion rate, so as to provide a natural, drug-free and healthy safety culture condition.本發明係提出一種用於無藥安全養殖的植物活性組合物及其萃取方法,其主要使用金銀花、黃柏、穿心連、山楂及甘草等藥材,其係經過水、乙醇及乙酸乙酯溶劑各進行一次萃取,再經濃縮混合後可以得到每一藥材的萃取物,這些藥材可以先混合後再萃取,或是先萃取後再混合,以獲得植物活性組合物。另外,亦可搭配大黃、黃耆或益生菌的使用。本發明利用抑菌的植物活性成分,並藉由萃取後的植物活性組合物的組合比例具有的協同增效的效應,不但可提高動物的免疫力,更可具有良好的飼料換肉率,以提供天然無藥健康的安全養殖條件。10‧‧‧第一濾液12‧‧‧第一殘渣14‧‧‧第一濃縮液16‧‧‧第二濾液18‧‧‧第二殘渣20‧‧‧第二濃縮液22‧‧‧第三濾液24‧‧‧第三濃縮液26‧‧‧萃取物