The present invention provides a bacteriostatic active plant composition and its extraction method, in which medicinal materials, such as honeysuckle, indigowoad root, root of membranous milk vetch, hawthorn, and licorice root, are subjected to extractions extraction by water, ethanol, and ethyl acetate solvent each for once, followed by concentration and mixture to obtain the extract of each of the medicinal materials. These medicinal materials can be mixed first and then extracted, or they can be extracted first and then mixed to obtain the active plant compositions. In addition, this invention can be used with wood anemone, barbed skullcap herb, or probiotics. This invention uses the bacteriostatic active plant compositions and the combination ratio of the extracted active plant compositions to achieve the effect of coordination enhancement, thereby to increase the immunity for animals and achieve the effect of bacteria resistance and inhibition and avoid the use of antibiotics or drugs, so as to provide a natural, drug-free and healthy safe culture condition.本發明係提出一種抑菌性植物活性組合物及其萃取方法,其主要使用金銀花、板藍根、黃耆、山楂及甘草等藥材,其係經過水、乙醇及乙酸乙酯溶劑各進行一次萃取,再經濃縮混合後可以得到每一藥材的萃取物,這些藥材可以先混合後再萃取,或是先萃取後再混合,以獲得植物活性組合物。另外,亦可搭配白頭翁、半支連或益生菌的使用。本發明利用抑菌的植物活性成分,並藉由萃取後的植物活性組合物的組合比例具有的協同增效的效應,以提高動物的免疫力,達到抗菌抑菌之功效而避免使用抗生素或藥物,以提供天然無藥健康的安全養殖條件。10‧‧‧第一濾液12‧‧‧第一殘渣14‧‧‧第一濃縮液16‧‧‧第二濾液18‧‧‧第二殘渣20‧‧‧第二濃縮液22‧‧‧第三濾液24‧‧‧第三濃縮液26‧‧‧萃取物