Derin C. Walters;Anna-Karin Maltais (formerly Anna-Karin Roos);Alan D. King;Britta Wahren;Kristian Haller (formerly Kristian Hallermalm);Andreas Brave;Robert J. Walters
发明人:
Richard E. Walters,Derin C. Walters,Alan D. King,Anna-Karin Maltais (formerly Anna-Karin Roos),Britta Wahren,Kristian Haller (formerly Kristian Hallermalm),Andreas Brave
申请号：
US13138722
公开号：
US20130123744A1
申请日:
2010.03.26
申请国别(地区):
US
年份:
2013
代理人:
摘要：
The invention provides a method and apparatus for the delivery of polynucleotide cancer vaccines to increase T cell response and reduce pain due to long electric waveform application and muscle contractions. The method includes the steps of: (a.) administering a polynucleotide cancer vaccine into the skin at an administration site, (b.) applying a needle electrode to the skin in the vicinity of the administration site, and (c.) applying a sequence of at least three electrical waveforms to deliver the polynucleotide cancer vaccine into skin cells by electroporation. The sequence has at least one of the following characteristics {1} at least two of the waveforms differ in amplitude, {2} at least two of the waveforms differ in width, and (3) a first waveform interval for a first set of two of the waveforms is different from a second waveform interval for a second set of two of the waveforms.
