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Culicinae Mosquito TRA-2 RNA Interference Technique to Genetically Produce Maleness Population
专利权人:
Duong Thanh Hoang
发明人:
Duong Thanh Hoang,Kim Phuc Hoang
申请号:
US15904548
公开号:
US20180334675A1
申请日:
2018.02.26
申请国别(地区):
US
年份:
2018
代理人:
摘要:
This invention entails a method to make a Tra-2 RNAi kernel sequence, and uses for this kernel sequence. The method includes the steps of amplifying an RNA recognition motive (RRM) DNA sequence from a Culcinae mosquito species, to obtain a first RRM DNA sequence of 240 base pairs then reversely connecting the first RRM DNA sequence with a second RRM DNA sequence via an intron or a linker DNA sequence, in such a way that the second RRM DNA sequence forms an inverted sequence to the first RRM DNA sequence. Transcription of the two DNA sequences produces single strands of mRNA that can bind together to form a double strand hairpin mRNA structure. Other steps include inserting a tetracycline repressible transactivator, and an insect spermatogenesis promoter.
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