METHODS AND MEANS FOR REMOVAL OF A SELECTED DNA SEQUENCE

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专利权人:

BAYER BIOSCIENCE N.V.

发明人:

D' HALLUIN KATHLEEN,RUITER RENE

申请号：

US20060910515

公开号：

US2009089890(A1)

申请日:

2006.03.31

申请国别(地区):

美国

年份:

2009

代理人:

摘要：

A method is described for the exact removal of a selected subfragment from a DNA molecule by intrachromosomal recombination between two directly repeated DNA sequences using a rare-cleaving double stranded break inducing DNA endonuclease expressed under control of a micro-spore specific promoter. This method can be applied in a method for the exact exchange of a target DNA fragment for a DNA fragment of interest in plant cells and plants.

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