The present invention provides a method for the production of a virus. The method includes providing a host cell that has been infected by the virus and cultivating the infected host cell at two different temperatures. The virus produced by the cultivation steps is subsequently collected. By using the dual temperature cultivation process, high titer and improved purity can be obtained.本發明係提供生產病毒的方法。該方法包括提供一宿主細胞，其已經被該病毒感染，並在兩個不同的溫度下培養該經感染之宿主細胞。隨後收集藉著該培養步驟產生之病毒。藉著使用雙重溫度培養製程，可獲得高力價和經改良之純度。
