WILLIAMS, JIM C,MONTGOMERY, MITZI M,NEWSOM, BRIAN S
申请号：
NZ57264407
公开号：
NZ572644A
申请日:
2007.05.04
申请国别(地区):
NZ
年份:
2012
代理人:
摘要：
Provided is a method of identifying one or more epitopes within a polypeptide antigen for an autoreactive T cell, comprising: (a) providing a sample comprising T cells isolated from a patient； (b) adding one or more different epitopes to a plurality of portions of the sample, wherein the sequences of the epitopes collectively comprise a portion of the sequence of the polypeptide antigen； and (c) identifying the one or more different epitopes that activate the patient's T cells. Further provided is a A method of preparing a personalized T cell vaccine, comprising (a) providing a sample comprising T cells isolated from a patient； (b) contacting the T-cells with one or more different epitopes, whereby autoreactive T cells are activated； (c) expanding activated autoreactive T cells； and (d) attenuating the autoreactive T cells； wherein the one or more different epitopes comprise all epitopes of an antigenic polypeptide capable of stimulating the patient's T cells with a stimulation index above a predetermined value.
