WILLIAMS, JIM C.,MONTGOMERY, MITZI M.,NEWSOM, BRIAN S.
申请号：
ES07797347
公开号：
ES2553192T3
申请日:
2007.05.04
申请国别(地区):
ES
年份:
2015
代理人:
摘要：
A method of preparing a T-cell vaccine, comprising: (a) providing a sample comprising T cells isolated from a patient； (b) adding peptide mixtures each comprising different peptides to a plurality of portions of the sample, wherein each different peptide in the peptide mixture is 8-20 amino acids and comprises an overlapping sequence of 4-19 amino acids with another different peptide of the peptide mixture； and wherein the sequences of different peptides in the peptide mixtures collectively comprise the complete sequence of a polypeptide antigen； and (c) identifying a part of the sample comprising activated autoreactive T cells, wherein a peptide mixture that activates the autoreactive cells identified by (c) with a stimulation index above a predetermined value is a specific peptide mixture of a patient； (d) provide an additional sample comprising T cells isolated from the patient； (e) contacting the T cells with the patient-specific peptide mixtures identified in steps (a) to (c), whereby the self-reactive T cells are activated； (f) expand activated autoreactive T cells； and (g) attenuate the autoreactive T cells, so that the T cells are incompetent for replication but still viable； wherein the stimulation index for each peptide mixture is calculated by comparing the incorporation of [3 H] thymidine into a sample of patient T cells cultured in the presence of a peptide mixture comprising a part of the polypeptide, with the incorporation of [3 H] thymidine in a sample of T cells cultured only in a control medium, which is expressed as the ratio of the average counts per minute of antigen aliquots / average counts per minute of the control aliquots, wherein the predetermined value is at least 1.3.Un método para preparar una vacuna de células T, que comprende: (a) proporcionar una muestra que comprende células T aisladas de un paciente； (b) añadir mezclas peptídicas que comprenden cada una diferentes péptidos a una pluralidad de porciones de la muestra, en donde cada
