The present disclosure provides a method for characterising a region of interest within or between biological tissue. The method comprises inserting at least a portion of a probe into the region of interest and directing electromagnetic radiation to the region of interest and receiving electromagnetic radiation from the region of interest using the probe in a direction that is transversal to a length of the probe. Further, the method comprises directing a fluid to the region of interest through a portion of the probe and analysing received electromagnetic radiation.
