A puncture device (1) comprises: a puncture instrument (10) for puncturing a biological tissue a longitudinally shaped urethral insertion member (4) that is to be inserted into the urethral lumen a longitudinally shaped vaginal insertion member (5) that is to be inserted into the vaginal lumen and a supporting member (2) supporting the urethral insertion member (4), the vaginal insertion member (5) and a puncture member (3) of the puncture instrument (10). The puncture instrument (10) comprises: the puncture member (3) that comprises a puncture needle (31) for puncturing a biological tissue, a shaft (33) and a connecting part (32) for connecting the puncture needle (31) and the shaft (33) and an indwelling assembly (8) that is located in the tip side of the puncture needle (31) and comprises a needle tip (82) for puncturing the biological tissue and an indwelling member (81) for supporting the biological tissue. The puncture needle (31) is tubular-shaped and has a hollow part (312). The indwelling member (81) for supporting the biological tissue is housed within the hollow part (312).Linvention concerne un dispositif de perforation (1) qui comprend : un instrument de perforation (10) pour perforer un tissu biologique un élément dintroduction urétrale longitudinalement façonné (4) qui doit être introduit dans la lumière urétrale un élément dintroduction vaginale longitudinalement façonné (5) qui doit être introduit dans la lumière vaginale et un élément de support (2) supportant lélément dintroduction urétrale (4), lélément dintroduction vaginale (5) et un élément de perforation (3) de linstrument de perforation (10). Linstrument de perforation (10) comprend : lélément de perforation (3) qui comprend une aiguille de perforation (31) pour perforer un tissu biologique, une tige (33) et une partie de liaison (32) pour relier laiguille de perforation (31) et la tige (33) et un ensemble à demeure (8) qui est situé dans le côté pointe de laiguille de perforation (31) e
