This invention relates to design of a novel antimicrobial cream for topical applications using an extract of tuberose flowers obtained by the green technology of supercritical carbon dioxide (SC- CO2) extraction. The extracts obtained by this technology at different extraction parameters have been assayed for phytochemical properties and antimicrobial potency against common pathogens. The extract with best combination of these properties was obtained at extraction conditions of 40°C, 100 bar and 135 min. This extract has been employed for formulation of a topical antimicrobial cream. No bacterial growth (signifying excellent antimicrobial potency) of Staphylococcus aureus was found in the cream formulated with extract at 5% (w/w of cream). This cream had the highest sensory appeal and antimicrobial potency comparable to two commercial topical antimicrobial creams.
