LIOTTA, LANCE A.,PETRICOIN, EMANUEL F.,GEHO, DAVID,ESPINA, VIRGINIA A.
申请号：
CA2667790
公开号：
CA2667790C
申请日:
2007.10.26
申请国别(地区):
CA
年份:
2014
代理人:
摘要：
This invention relates, e.g., to a composition that, at room temperature, whencontacted with a sample comprisingphosphoproteins, can fix and stabilize cellular phosphoproteins, preservecellular morphology, and allow the sample to be frozen togenerate a cryostat frozen section suitable for molecular analysis. Thecomposition comprises ( 1 ) a fixative that is effective to fixthe phosphoproteins, and that has a sufficient water content to be soluble fora stabilizer and/or a permeability enhancing agent)； (2) astabilizer, comprising (a) a kinase inhibitor and (b) a phosphatase inhibitorand, optionally, (c) a protease (e.g., proteinase) inhibitor；and (3) a permeability enhancing agent (e.g. PEG). Methods are described forpreserving phosphoproteins, using such a composition.Also described are endogenous surrogate markers for monitoring proteindegradation, including the loss of posttranslationalmodifications (such as phosphorylation), e.g. the following removal of a cellor tissue from a subject； and exogenous molecularsentinels (e.g. phosphoproteins attached to magnetic nanoparticles) that allowone to evaluate the processing history of a cellular ortissue population sample.
