Provided is a fatty acid composition containing linoleic acid, linolenic acid and oleic acid. Also provided is a fatty acid composition containing linoleic acid, linolenic acid and oleic acid, as well as at least one selected from palmitinic acid, palmitoleic acid, stearic acid, arachidic acid and docosanoic acid. Also provided are a plant extract and a pharmaceutical preparation, wherein the pharmaceutical preparation contains an active component, and the active component comprises at least one of the fatty acid composition, the plant extract and modified products thereof. In addition, also provided is an application of the fatty acid composition, the plant extract and the pharmaceutical preparation in multiple fields. The pharmaceutical preparation according to the present invention functions to repair various wounds and traumas in skin, mucosa, lumina and muscular tissues, and has the advantages of rapid healing, no infection, no scar and no formation of purulent tissues, and has such good effects as removal of tissue exudate, removal of inflammation and infection, and promoting repairing and healing of wound muscle and skin tissues.