#$%^&*AU2020101687A420200924.pdf#####CN11253579A Abstract Page 1/1 The present invention relates to the field of biomedicine and relates to a preparation method for a dextran-hyaluronic acid hydrogel for three-dimensional cell culture and application thereof. The hydrogel freeze-drying scaffold synthesized by the present invention has interconnected pores and has pore size and porosity suitable for cell growth. In-vitro experiments show that cells are inoculated and cultured in the hydrogel which is non-toxic and has good cell compatibility, and the cells have a high survival rate in the hydrogel. One month after the hydrogel is injected subcutaneously into mice, the phenomena of inflammation and vascular proliferation do not occur, and the hydrogel is easily degraded without toxic or side effect. In-vivo experiments show that the hydrogel is injected subcutaneously into mice after being inoculated with cells, which can keep the cells in a favorable growth status, form tumor massive tissues and provide a necessary three-dimensional growth environment for the cells. The hydrogel can replace extracellular matrices functionally, and provide a scaffold and a three-dimensional growth environment for growth and attachment of cells. As a clinical in-vivo filling material, the hydrogel has the advantages of high bio-safety, simple and convenient operation, and low price. 1CN111253579A Drawings of Description Page 1/7 1:44:31 PM 20.00 kV 3.0 10.0 mim 1 000 x SE Nova NanoS[ M FIG. 1 14
