A method of detecting invasive fungi according to morphology thereof based on contrast staining, including: sterilizing and storing the necessary equipment aseptically； drawing 1 ml of venous blood from a tested subject's elbow vein； dripping one drop of the venous blood, prior to coagulation, into an ampoule containing 0.8 ml of a detection reagent under an aseptic environment； gently shaking the ampoule until the drop of venous blood is evenly distributed； leaving the ampoule to stand for 20 minutes to form a stained solution； sterilizing or disinfecting a microscope slide and a cover slip； dripping one drop of the stained solution on the microscope slide prepared under aseptic condition； observing the sample sequentially with 4×, 10× and 40× objective lenses and a 100× oil-immersion lens； magnifying with a 5 million pixel eyepiece； displaying an image of the sample on computer screen using a high-resolution imaging software for observation and record.
