What is provided is an EPO mimetic peptide derivatives defined as formula (I)and itspharmaceutical salt, the preparation thereof, wherein R1, R2, R3, R4, R5, n1,n2 aredefined as described in description. A composition comprising of an EPOmimeticpeptide derivatives defined as formula (I) and its pharmaceutical salt. Theuses of thederivatives and its pharmaceutical salt, as well as the uses of thecomposition describedabove in treatment of diseases characterized by a deficiency of EPO or a lowordefective red blood cell population.R1-R2-(CH2)n1-R3-(CH2)n2-R4-R5(I)