KIM, DAE JIN,金大振,金大振,LEE, BYUNG SUN,李炳善,李炳善,HONG, SUNG HWAN,洪性焕,洪性煥,HUH, YONG HO,许容豪,許容豪,JUNG, SUNG YOUB,郑圣烨,鄭聖燁,KWON, SE CHANG,权世昌,權世昌
申请号：
TW101136851
公开号：
TW201331223A
申请日:
2012.10.05
申请国别(地区):
TW
年份:
2013
代理人:
摘要：
The present invention relates to a blood coagulation factor VII derivative, a blood coagulation factor VIIa derivative, FacVII and FacVIIa conjugates each prepared by linking a polymer capable of extending the blood half-life to the derivative, FacVII and VIIa complexes each prepared by linking a carrier to the conjugate, genes encoding the FacVII and FacVIIa derivatives, expression vectors comprising the genes, transformants introduced with the expression vectors, a method for preparing the FacVII and FacVIIa derivatives using the transformants, a method for preparing the FacVIIa conjugate and complex, a FacVIIa complex prepared by the method, a pharmaceutical composition for the prevention or treatment of hemophilia comprising the derivative, conjugate, or complex as an active ingredient, and a pharmaceutical composition for blood coagulation comprising the derivative, conjugate, or complex as an active ingredient. Further, the present invention relates to a method for preventing or treating hemophilia or for promoting blood coagulation, comprising administering to a subject a therapeutically effective amount of the composition. The FacVII or FacVIIa derivative of the present invention is able to bind with a carrier capable of improving the blood half-life while maintaining the activity of FacVII or FacVIIa, and they can be widely used in the development of effective prophylactic or therapeutic agent for hemophilia.本發明係有關於凝血因子Ⅶ衍生物,凝血因子Ⅶa衍生物,FacⅦ及FacⅦa接合物(各經將可延長血液半衰期之聚合物連結至該衍生物而製備),FacⅦ及Ⅶa複合物(各經將載劑連結至該接合物而製備),編碼該FacⅦ及FacⅦa衍生物之基因,包含該基因之表現載體,經導入該表現載體之轉形株,使用該轉形株製備FacⅦ及FacⅦa衍生物之方法,製備FacⅦa接合物及複合物之方法,藉由該方法製備之FacⅦa複合物,包含該衍生物、接合物或複合物作為活性成分之用於預防或治療血友病的醫藥組合物,以及包含該衍生物、接合物或複合物作為活性成分之凝血用醫藥組合物。再者,本發明係有關於用於預防或治療血友病或促進凝血的方法,包含投予個體治療有效量之該組合物。本發明之FacVII或FacVIIa衍生物能與可改良血液半衰期的載劑結合,同時維持FacVII或FacVIIa的活性,且它們可廣泛用於發展血友病的預防劑或治療劑。該代表圖無元件符號及其代表之意義。
