A microRNA-based short hairpin RNA for gene knockdown of the N-methyl-D-aspartate receptor NR1 subunit, comprises a first fragment, a second fragment and a joining fragment. The second fragment is a palindrome with an inverted repeat sequence of the first fragment. The joining fragment composed by bases at random, and the two ends of the joining fragment connect with the first and second fragment, respectively. The first fragment comprises a dicer-cutting site, and a Drosha-cutting site, and between the two sites is a silencing fragment. The silencing fragment is homologous to the RNA sequence of NMDA receptor NR1 subunit. The microRNA-based short hairpin RNA has a sequence set forth in SEQ ID NO: 5 or 6. The present invention also discloses the use of the microRNA-based short hairpin RNA and a medication to release skin inflammatory pain.一種用以抑制NMDA受體NR1之以微小RNA為基礎之短夾RNA,係由一單股核糖核酸形成,該單股核糖核酸係包含一第一片段、一第二片段及一連接片段,該第二片段係與該第一片段互補之回文序列,該連接片段為任意排列之鹼基序列,該連接片段之兩端分別連接該第一片段及該第二片段,其中該第一片段係具有一裁切子切位及一Drosha切位,該裁切子切位及該Drosha切位之間包含一默化片段,該默化片段係與NMDA受體次單元NR1同源,其中,該以微小RNA為基礎之短夾RNA係如SEQ ID NO:5或6所示之序列。本發明另揭示該以微小RNA為基礎之短夾RNA之用途及其醫藥組合物。11‧‧‧第一片段111‧‧‧Drosha切位112‧‧‧裁切子切位113‧‧‧默化片段12‧‧‧第二片段13‧‧‧連接片段
