Flooring challenge systems and processes for culling poultry reared in pens by adding torque, stress and strain on key leg joints in to induce lameness attributable to osteochondrosis and osteomyelitis of the proximal femur and tibia. The flooring challenge systems utilize one or more portable panels or sections that can be constructed in a wide range of sizes and configurations for installation in commercial poultry pens. In addition, the flooring challenge systems may utilize a device suspended a predetermined distance above the apex of the flooring challenge panel to subject the poultrys legs to asymmetric twisting and enhanced instability by forcing the birds to straddle opposing slopes near the apex of the flooring challenge panel. After a suitable incidence of lameness has been induced, the panels or sections of the flooring challenge system can be removed to facilitate cleanout of the rearing pens.