Flooring challenge systems for culling poultry that induce lameness attributable to osteochondrosis and osteomyelitis of the proximal femur and tibia in poultry. The flooring challenge systems induce unstable or insecure footing for poultry reared in pens by adding torque, stress and strain on key leg joints in order to exacerbate and accelerate the development of bacterial chondronecrosis with osteomyelitis lesions and lameness. The flooring challenge systems utilize one or more portable panels or sections that can be constructed in a wide range of sizes and configurations for installation in commercial poultry pens. In addition, the flooring challenge systems may utilize a device suspended a predetermined distance above the apex of the flooring challenge panel to subject the poultrys legs to asymmetric twisting and enhanced instability by forcing the birds to straddle opposing slopes near the apex of the flooring challenge panel.
