PROBLEM TO BE SOLVED: To provide an efficient detection method or production method of a spore-deficient variant in an organism of a basidiomycete subphylum.SOLUTION: A detection method of a spore-deficient variant includes a step of detecting an existence of mutation or deficiency in one or more biomolecule selected from the group consisting of (a) DNA chain consisting of a base sequence shown by a sequence number 1, (b) homologous gene of mismatch repair gene, (c) RNA chain, cDNA, or protein derived from the DNA chain in the (a), and (d) RNA chain, cDNA, or protein derived from homologous gene of the (b), which are derived from an organism of a basidiomycete subphylum.COPYRIGHT: (C)2014,JPO&INPIT【課題】 担子菌亜門の生物の胞子欠損性変異株の、効率的な検出方法又は生産方法。【解決手段】 担子菌亜門の生物由来の、(a)配列番号1で示される塩基配列からなるDNA鎖、(b)ミスマッチ修復遺伝子の相同遺伝子、(c)前記(a)のDNA鎖由来のRNA鎖、cDNA、もしくは蛋白質、及び(d)前記(b)の相同遺伝子由来のRNA鎖、cDNA、もしくは蛋白質、からなる群から選ばれる1つ以上の生体分子の、変異又は欠損の有無を検出する工程を含む、胞子欠損性変異株の検出方法を用いる。【選択図】 図9