The present invention relates to an underwater seed plasma treatment method. The underwater seed plasma treatment method according to one embodiment of the present invention generates discharge plasma in a culture solution including a seed. According to the embodiment of the present invention, the underwater seed plasma treatment method uses a pulse discharge device for pulse underwater plasma discharge.본 발명은, 종자가 포함된 배양액에 방전 플라즈마를 발생시켜 종자 및 종자 배양액을 플라즈마 처리 방법에 관한 것이다.