A method for inhibiting a cellular glycolysis process and an application thereof. The method is using reductive selenium dioxide, selenite or formic acid, formate, oxalic acid, oxalate, sulfur-containing reducing agent, and the like to inhibit a hydrogen cyclic process required in a redox reaction in the cellular glycolysis process. The application comprises: tumor prevention and treatment by applying different reducing agents by means of injection, oral administration, external application, spraying, or fumigation; prevention and treatment of digestive tract inflammation and tumors by means of oral administration; inhibition of a metabolic process of anaerobes and facultative anaerobes, where the reductive selenium dioxide, selenite, formic acid, formate, and the like are used as one of the active ingredients or ingredients of a fungicide; and disease prevention and antioxidation of fresh goods, food, fish and meat, vegetables and fruits, natural perfume, natural cosmetics, detergents, skin care products, an
