МАКДОНАЛЬД Линн (US),СТИВЕНС Шон (US),МЕРФИ Эндрю Дж. (US)
申请号：
RU2012128856/10
公开号：
RU2012128856A
申请日:
2010.12.10
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A mouse comprising the genetic modification of germline, which comprises a deletion of the nucleotide sequence encoding the CH1 IgG, wherein the mouse expresses an IgM, which comprises a CH1 domain, and the mouse expresses the antibody in serum IgG, which lacks the CH1 domain, and wherein the light chain is absent, and wherein mouse IgG is not capable of expressing mRNA which comprises a sequence encoding domain CH1.2. The mouse of claim 1, wherein the deletion of the nucleotide sequence encoding the CH1, stored in IgG1.3 gene. The mouse of claim 1 which comprises Functional gene light chain locus immunoglobulina.4. The genetically modified mouse of claim 1, wherein the antibody is IgG, which lacks the light chain comprises a human variable domain and constant domain of the mouse, which lacks CH1.5 domain. The genetically modified mouse of claim 1, wherein the antibody is IgG, which lacks the light chain contains mouse variable domain and constant domain of the mouse, which lacks CH1.6 domain. The genetically modified mouse of claim 1 which comprises a deletion IgG2a.7 gene. The genetically modified mouse of claim 1 which comprises a deletion IgG2b.8 gene. The genetically modified mouse of claim 1 which comprises a deletion of the gene and a deletion IgG2b.9 IgG2a gene. The genetically modified mouse of claim 1 which comprises a deletion of the gene and a deletion IgG2b.10 IgG2a gene. The genetically modified mouse of claim 1, which contains a substitution of one or more endogenous variable region gene segments of the mouse heavy chain one or more variable region gene segments of the heavy chain cheloveka.11. The genetically modified mouse of claim 10, wherein one or more gene Seg1. Мышь, содержащая генетическую модификацию зародышевой линии, которая включает делецию нуклеотидной последовательности, кодирующей CH1 IgG, где мышь экспрессирует IgM, который содержит домен CH1, и мышь экспрессирует в сыворотку антитело IgG, в котором отсутствует домен CH1 и в котором
