PROBLEM TO BE SOLVED: To provide an antibody against cancer stem cell markers for diagnosing and treating solid tumor.SOLUTION: This invention relates to an isolated antibody which specifically binds to an extracellular domain of a human DLL4 formed by combining a human DLL4N terminal region, which is a human DLL4 epitope, and a human DLL4DSL, and which affects proliferation of a tumor including a cancer stem cell. The invention also relates to a method for producing an antibody which comprises transforming a host cell with a polynucleotide expression vector encoding an antibody, expressing the antibody, and purifying the antibody from a culture medium. Furthermore, the invention relates to a method for diagnosing and treating cancer with the antibody.SELECTED DRAWING: None【課題】充実性腫瘍の診断および処置のための癌幹細胞マーカーに対する抗体の提供。【解決手段】ヒトDLL4エピトープであるヒトDLL4N末端領域、およびヒトDLL4DSLの組み合わせによって形成されるヒトDLL4の細胞外ドメインに特異的に結合し、かつ、癌幹細胞を含む腫瘍の増殖に影響を与える単離抗体。また、抗体をコードするポリヌクレオチド発現ベクターで宿主細胞を形質転換し、抗体を発現させ、培養培地から抗体を精製する抗体の製造方法。更に、該抗体による癌の診断方法、及び癌を処置する方法。【選択図】なし
