Robbie Woodman,Johannes Tsung-Han Yeh,Sophie Laurenson,Paul Ko Ferrigno
申请号：
US13243318
公开号：
US08481491B2
申请日:
2011.09.23
申请国别(地区):
US
年份:
2013
代理人:
摘要：
The present invention relates to the use of Stefin A as a scaffold protein for the display of inserted peptides, particularly wherein the Stefin A is a human Stefin A. Several mutations are advantageously made in the wild type stefin A sequence to improve it as a scaffold preferably the Stefin A comprises a heterologous peptide insertion at the Leu 73 site. Furthermore, preferably the scaffold protein comprises a V48D mutation preferably the scaffold protein comprises a G4W mutation. Preferably the scaffold comprises Leu73, V48D and G4W mutations. The invention also relates to the scaffold proteins themselves, in particular a stefin A polypeptide having the Leu73, V48D and G4W mutations, such as shown as SEQ ID NO: 1. The invention also relates to a method for identifying binding proteins and to peptide A (RLNKPLPSLPV, SEQ ID NO: 30) and its use in treating yeast infections.
