CHIEN, MAW SHENG,简茂盛,簡茂盛,HUANG, CHIEN JIN,黄千衿,黃千衿,WU, CHI MING,吴志铭,吳誌銘
申请号：
TW103126503
公开号：
TWI577802B
申请日:
2014.08.01
申请国别(地区):
TW
年份:
2017
代理人:
摘要：
The present invention relates to a method for producing extracellular secretory porcine circovirus type 2 capsid protein virus-like particles by using baculovirus expression system, insect cell and serum-free culture medium. The FBS-free PCV2 recombinant Cap protein can be recognized by anti-PCV2 antibodies, and self-assembly into virus-like particles for using as antigen in preparing subunit vaccines.本發明係關於利用桿狀病毒表現系統、昆蟲細胞及無血清培養液,製備胞外分泌型豬環狀病毒全長外殼蛋白(PCV2 Cap)的類病毒顆粒(VLP)。本發明製備得之不含胎牛血清的胞外分泌型豬環狀病毒重組Cap蛋白,不僅可被特異性抗-PCV2抗體所辨認,更可自我組裝成類病毒顆粒,故極具適合做為次單位疫苗之疫苗抗原之潛力
