The invention relates to a process for decreasing the amount of cholesterol ina mixture comprising a marine oil, the marine oil containing the cholesterol,which process comprises the steps of adding a volatile working fluid to themixture, where the volatile working fluid comprises at least one of a fattyacid ester, a fatty acid amide and a hydrocarbon, and subjecting the mixturewith the added volatile working fluid to at least one stripping processingstep, in which an amount of cholesterol present in the marine oil in free formis separated from the mixture together with the volatile working fluid. Thepresent invention also relates to a volatile cholesterol decreasing workingfluid and a health supplement and a pharmaceutical, based on a marine oil,prepared according to the process mentioned above.