PROCEDE DE PRODUCTION D'UN VECTEUR DE VIRUS A ARN A BRIN NEGATIF A L'AIDE D'UN PROMOTEUR HYBRIDE CONTENANT UN ACTIVATEUR DE CYTOMEGALOVIRUS ET UN PROMOTEUR DE .BETA.-ACTINE AVIAIRE
The present invention provides methods for producing a minus-strand RNA viral vector, which comprise using a promoter comprising a cytomegalovirus enhancer and a chicken .beta.-actin promoter, to induce the transcription of the genome RNA of a minus-strand RNA viral vector and the expression of minus-strand RNA viral proteins that form a ribonucleoprotein with the genome RNA. The methods of the present invention enable high efficiency production of highly safe minus-strand RNA viral vectors. The methods of the present invention are particularly useful for producing minus-strand RNA viral vectors that are deficient in envelope-constituting protein genes.
