The present invention pertains to engineered extracellular vesicles(EVs) for use in a variety of research, diagnostic, imaging and therapeutic applications. The EVs are engineered to enable incorporation and surface display of various proteins of interest, which may be exogenous and/or endogenous in nature. The coating of EVs is achieved through inventive protein engineering of EV polypeptides, and the present invention thus relates to methods for coating of EVs, EVs per se, as well kits, detection methods, diagnostic applications, imaging and delivery methods based on said engineered EVs.
