Provided is a fluorescence observation apparatus whereby the dependency on remaining distance and the like in a divided image can be adequately removed and observation can be carried out using a highly quantitative fluorescence image. Adopted is a fluorescence observation apparatus (100) provided with: a pre-processing unit (46) for multiplying a fluorescence image and/or reference light image by a coefficient whereby the distance characteristic of return light intensity and the distance characteristic of fluorescence intensity acquired in advance for a standard sample reach a relationship of direct proportionality to each other, to generate a correction fluorescence image and a correction reference light image a division image generation unit (43) for dividing the correction fluorescence image by the correction reference light image to generate a division image a threshold value setting unit (45) for setting a threshold value on the basis of the mean value of the grayscale value of every pixel in the division image an image adjustment unit (51) for expanding the contrast in the division image between a region having a grayscale value higher than the set threshold value and a region having a grayscale value lower than the threshold value and a monitor (50) for displaying the division image in which the contrast has been expanded.Linvention concerne un appareil dobservation de la fluorescence grâce auquel la dépendance à la distance restante et des paramètres similaires dans une image divisée peuvent être adéquatement éliminés et lobservation peut être effectuée en utilisant une image de fluorescence hautement quantitative. Linvention concerne un appareil dobservation de la fluorescence (100) muni dune unité de prétraitement (46) pour multiplier une image de fluorescence et/ou une image de lumière de référence par un coefficient par lequel la distance caractéristique de lintensité de lumière de réponse et la distance caractéristique de lintensité de fluorescence acqu
