A perimeter defense assembly for preventing burrowing and ambulatory intruders from entering an area includes a control housing coupled to a building. A processor is coupled to the control housing. An inverter is coupled to the control housing. The inverter is operationally coupled to the processor. A base is positioned beneath a support surface. A support is coupled to the base. The support extends above the support surface. A wire is coupled to the support. The wire encompasses an area. The wire is operationally coupled to the processor. The wire is energized so the wire may repel an intruder.