A collection bottle (A1) and a method for detecting a characteristic of a sample are provided. The collection bottle (A1) includes a body (10) and a cover (20). The body of the collection bottle (A1) has an accommodating space (14). The cover (20) of the collection bottle (A1) is removably assembled to the body (10) of the collection bottle (A1), and the cover (20) includes a channel (21) and a testing part (22). The channel (21) of the collection bottle (A1) communicates the accommodating space (14) and the outside environment. While the cover (20) of the collection bottle (A1) is assembled to the body (10), the testing part (22) extends toward the accommodating space (14).