The present invention discloses an apparatus for detecting analyte in a liquid sample, comprising a cup body； a first receiving area for receiving a liquid sample； a flow-guiding channel through which a sample can be added or collected； the flow-guiding channel is in communication with the first receiving area, the bottom surface of the flow-guiding channel is a slope； wherein, the first receiving area and the flow-guiding channel are disposed in the cup body； the apparatus further comprises a secondary sampling port； the flow-guiding channel is a groove, in which is provided with a second receiving area； and the second receiving area includes a corner area for collecting samples for secondary sampling. The present invention further provides a method of using the apparatus for detecting an analyte in a liquid sample. The apparatus of the present invention can be used for detecting the presence or amount of an analyte in a liquid sample. When a liquid sample has extremely poor fluidity and/or the sample size is very small, the apparatus is still capable of detecting liquid samples, to facilitate operators to draw liquid samples for second confirmatory detection.
