PROCEDE DE PRODUCTION A GRANDE ECHELLE DESTINE A DES CYSTEINES D'ANTICORPS COIFFEES ET NON COIFFEES ET LEUR UTILISATION DANS LA CONJUGAISON DE PROTEINES THERAPEUTIQUES
DUTTA, KAUSHIK,GOMES, JOSE MANUEL,KOTCH, FRANK W.,PATEL, VIMALKUMAR B.,PRASHAD, AMARNAUTH SHASTRIE,PROCOPIO-MELINO, RENEE L.,ZHONG, XIAOTIAN
申请号：
CA3052539
公开号：
CA3052539A1
申请日:
2018.02.01
申请国别(地区):
CA
年份:
2018
代理人:
摘要：
Optimizing production of selectively capped, and uncapped, cysteines on antibodies by manipulation of cell growth conditions including the deliberate depletion of cysteine and/or cystine in the cell culture process by way of media components, batch duration, or cell density to achieve efficient production of proteins including antibody-drug-conjugates (ADCs).； conjugating a TNB-capped cysteine-containing protein by reacting it with a reducing agent capable of detaching the TNB-capping moieties from the protein without significantly reducing antibody inter-chain sulfur bonds, and conjugating reduced sulfur bonds on the protein to a payload through a reactive linking moiety.
