Kaushik DUTTA,Jose Manuel GOMES,Frank W. KOTCH,Vimalkumar B. PATEL,Amarnauth Shastrie PRASHAD,Renee L. PROCOPIO-MELINO,Xiaotian ZHONG
申请号：
US16480792
公开号：
US20190381182A1
申请日:
2018.02.01
申请国别(地区):
US
年份:
2019
代理人:
摘要：
Optimizing production of selectively capped, and uncapped, cysteines on antibodies by manipulation of cell growth conditions including the deliberate depletion of cysteine and/or cystine in the cell culture process by way of media components, batch duration, or cell density to achieve efficient production of proteins including antibody-drug-conjugates (ADCs)； conjugating a TNB-capped cysteine-containing protein by reacting it with a reducing agent capable of detaching the TNB-capping moieties from the protein without significantly reducing antibody inter-chain sulfur bonds, and conjugating reduced sulfur bonds on the protein to a payload through a reactive linking moiety.
