A two-stage hundred-microsecond pulse focused ultrasound tissue destruction method, comprising: 1) positioning a target tissue of a sample (9) by using a monitoring guidance system, and adjusting a position of the target tissue to a focal point of an HIFU transducer (5) 2) a first stage destruction: forming loose local tissue structures by means of a shock wave excited by a hundred-microsecond pulse focused ultrasound, as well as boiling air bubbles and inertial cavitation generated thereby 3) a second stage destruction: further mechanically crushing and homogenizing the tissues in a damaged area by means of a pulse focused ultrasound having a hundred-microsecond length, so as to achieve tissue destruction. The method takes full advantage of the following features: a pulse having a relatively high duty cycle exhibits both thermal and mechanical effects, while a pulse having a low duty cycle exhibits good mechanical effects. By controlling a high-intensity ultrasound sequence in which two stages are generated consecutively, a single pulse of which lasts one hundred microseconds, and which has a pulse having a relatively high duty cycle and a low duty ratio to act on a target tissue, highly efficient tissue destruction may be achieved.La méthode de destruction de tissus par ultrasons focalisés par impulsion de centaines de microsecondes à deux étages comprend: 1) le positionnement du tissu cible dun échantillon (9) à laide dun système de guidage de surveillance, et lajustement de la position du tissu cible au point focal dun transducteur HIFU (5) 2) la première étape de destruction consiste à former des structures tissulaires locales lâches au moyen dune onde de choc excitée par une impulsion de centaines de microsecondes focalisée, ainsi que des bulles dair débullition et une cavitation inertielle 3) la seconde étape de destruction consiste à broyer et à homogénéiser mécaniquement les tissus de la zone endommagée au moyen dultrasons focalisés par impulsions ayant une
