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Resistance to multiple viruses in plants
专利权人:
Monsanto Technology LLC
发明人:
HUANG, Shihshieh,FLASINSKI, Stanislaw,FRIZZI, Alessandra,GABOR, Brad,HAGEN, Charles,KAO, John,SALATI, Raquel
申请号:
ES10718360
公开号:
ES2651909T3
申请日:
2010.04.20
申请国别(地区):
ES
年份:
2018
代理人:
摘要:
A transgenic tomato plant or a transgenic tomato seed, or a transgenic tomato plant cell comprising resistance to plant virus species of at least two of the Geminiviridae, Bunyaviridae and Flexiviridae families, in which resistance is provided by the minus two different modes of action selected from (a) expression of a nucleic acid construct that produces interfering dsRNA with the expression of a virus envelope protein gene, a virus movement protein gene, or a virus replication; (b) expression of a nucleic acid construct that produces interfering miRNA with the expression of a virus coat protein gene, a virus movement protein gene, or a virus replication gene; and (c) expression of a terminal sequence of the genomic segment of tospovirus that inhibits virion assembly, in which the resistance provided against at least one of the plant virus species is provided by the expression of a terminal repeat sequence of the Tospovirus genomic segment that inhibits tospovirus virion assembly, wherein in addition the tospovirus genomic terminal repeat sequence is selected from a nucleic acid sequence comprising SEQ ID NO: 167, a nucleic acid sequence comprising the SEQ ID NO: 168, a nucleic acid sequence comprising SEQ ID NO: 376, a nucleic acid sequence comprising SEQ ID NO: 377, a nucleic acid sequence comprising SEQ ID NO: 378, and a sequence of nucleic acid comprising SEQ ID NO: 455.Una planta transgénica de tomate o una semilla transgénica de tomate, o una célula vegetal transgénica de tomate que comprende resistencia a especies de virus de plantas de al menos dos de las familias Geminiviridae, Bunyaviridae y Flexiviridae, en la que la resistencia se proporciona por al menos dos modos diferentes de acción seleccionados de (a) expresión de una construcción de ácido nucleico que produce ARNbc interferente con la expresión de un gen de la proteína de la envuelta del virus, un gen de la proteína del movimiento del virus o un gen de la replicación del virus; (b) expresión de un
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