Disclosed is a process for the preparation of a non-viable probiotic composition having anti-allergic (i.e. allergy preventive and/or anti-allergic in a therapeutic sense, and/or anti-inflammatory properties, the process comprising the steps of (a) subjecting LGG to cultivation in a suitable culture medium using a batch process； (b) harvesting the culture supernatant at a late exponential phase of the cultivation step； (c) removing liquid contents from the culture supernatant so as to obtain the composition. Also disclosed is a composition comprising a proteinaceous mixture obtainable by the above process, and the use thereof in a dietetic product preferably for targeting expecting mother's, infants, or children as well as application in food for specific medical purposes.
