A method for producing a rooted cutting which can be applied to Pinaceae trees. Specifically, the present invention provides a method for producing a rooted cutting of a Pinaceae tree, comprising the following steps A and B: A: adding a cytokinin to the outside of a shoot apex of a short shoot of a Pinaceae tree to thereby induce a new scaly leaf and/or primary leaf； and B: cutting out the short shoot where new scaly leaf and/or primary leaf is induced in the step A, inserting the short shoot as a cutting in a rooting bed wetted with a carbon source-free liquid medium comprising nitrogen, phosphorus and potassium as essential elements, and culturing the short shoot in a culturing vessel while regulating the concentration of CO2 gas in the culturing vessel at a humidity of 80% or more to root the cutting.
