A method for incubating fruiting bodies of Antrodia cinnamomea is disclosed. The method comprises steps of: (a) obtaining a hymenium slice from a fruiting body of Antrodia cinnamomea (S1) (b) transferring the hymenium slice to a selective culture medium for incubation to obtain an isolated strain (S2) (c) transferring the isolated strain to a bagasse culture medium for incubation (S3) (d) subjecting proliferation by liquid culture or solid culture to obtain large-scale liquid spawn or solid spawn (S4) (e) inoculating a wood segment with the liquid spawn or the solid spawn and subjecting incubation (S5) and (f) re-inoculating the wood segment with mixed single-spore colonies of Antrodia cinnamomea and subjecting incubation until fruiting bodies are produced (S6).本案為一種培養牛樟芝子實體之方法,其包含步驟:(a)自一牛樟芝子實體取得一子實層切片;(b)將該子實層切片置於一選擇性培養基中進行培養,俾得到一純化菌種;(c)將該純化菌種置於一蔗渣培養基中進行培養,俾形成一增殖菌種;(d)將該增殖菌種進行液態或固態之增殖培養,俾得到大量液態或固態菌種;(e)將該液態或固態菌種接種至一段木上進行培養;以及(f)再次於該段木上接種混合單孢菌落,並培養至子實體出現。
