Kai Krohn,Vesna Blazevic,Marja Tähtinen,Mart Ustav,Urve Toots,Andres Männik,Annamari Ranki,Ene Ustav
申请号：
US14153576
公开号：
US09725486B2
申请日:
2014.01.13
申请国别(地区):
US
年份:
2017
代理人:
摘要：
A method for treating an HIV disease in a subject in need of said treatment, comprising administering to the subject a therapeutically effective amount of a DNA vaccine comprising an expression vector and a pharmaceutically acceptable excipient, where the expression vector comprises: (a) a heterologous promoter operatively linked to a DNA sequence encoding a nuclear-anchoring protein, where the nuclear-anchoring protein comprises: (i) a DNA binding domain which binds to a specific DNA binding sequence, and (ii) a functional domain of the Bovine Papilloma Virus Type 1 E2 protein, where the functional domain binds to a nuclear component (b) a multimerized DNA sequence that forms a binding site for the nuclear anchoring protein and (c) at least one expression cassette comprising a DNA sequence encoding a protein or peptide that stimulates an immune response specific to the protein or peptide where the expression vector lacks an origin of replication functional in mammalian cells.
