A method of antibacterial treatment of a carcass (26) in a carcass handling installation. The method comprises: conveying the carcass (26) through a treatment tunnel (2), and spraying a liquid cryogen in to the tunnel (2) so as to form a cryogenic liquid mist comprising cryogenic liquid droplets that impinge on the carcass (26) while the carcass (26) is in the tunnel (2). The cryogenic liquid mist is sprayed into the tunnel (2) so as to subject the carcass (26) to temperatures below substantially 0°C for a duration between about 5 seconds and about 60 seconds.