BROWNING, JEFFREY,MIATKOWSKI, KONRAD,MEIER, WERNER
申请号：
HK01108887.8
公开号：
HK1038244B
申请日:
2001.12.19
申请国别(地区):
HK
年份:
2012
代理人:
摘要：
The invention relates to methods for the expression of high yields of the form protein-immunoglobulin fusions having high affinity binding to its ligand, by culturing hosts transformed with DNA encoding the desired fusions in a cultutre system at a low temperature thereby minimizing the amount of misfolded or misbridged protein forms. Said host cells may be transformed mammalian, insect, yeast or bacterial cells. The protein-immunoglobulin fusion may comprises a member of the TNF or TNF-receptor family, such as lymphotoxin-beta or lymphotoxin-beta receptor. The invention also relates to pharmaceutical composition comprising said protein-immunoglobulin fusions.L"invention concerne une méthode qui permet d"obtenir un haut niveau d"expression de protéines chimères immunoglobulines actives réceptrices de la lymphotoxine-bêta. L"invention concerne également une méthode purification desdites protéines.
