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PRODUCCION RECOMBINANTE DE PEPTIDOS
专利权人:
BASF SE
发明人:
HUMMERICH, DANIEL,LIEBMANN, BURGHARD,FEHR, MARCUS,SCHWALB, CARSTEN,BRUSER, HEIKE
申请号:
PE0020492011
公开号:
PE10332012A1
申请日:
2010.06.02
申请国别(地区):
PE
年份:
2012
代理人:
摘要:
For a precursor protein which comprises a Repetitive sequence, which can be cleaved from the elements of the desired Peptide (PEP) and auxiliary (aux) Peptide elements of the formula Company (PEP (aux aux) x, - X, PEP)Where X > 1 the elements are identical or different, and they include elements of amino acid sequences that provide the precursor protein self-assembly properties WBS elements are identical or different and comprise the amino acid sequences of Peptide molecules IdenTIC As different and comprise an Amino Acid Sequence SEQ ID no: 20 selected or SEQ ID no:29 to 67.The WBS elements and Peptide Bonds form between the if and the Peptide bond is specifically Chemically or enzymatically cleavable Peptide In addition, at least one auxProtective Element comprises a Peptide (a) which has an increased portion of negatively charged amino acid residues capable of forming a helical Structure anfifilicaseleccionada amino acid sequences SEQ ID no: 16 to SEQ ID no: 19 and SEQ ID no: The precursor protein is Produced by a recombinant Strain of E.Coli and is useful in the production of peptides by BiotechnologyREFERIDA A UNA PROTEINA PRECURSORA CARACTERIZADA PORQUE COMPRENDE UNA SECUENCIA REPETITIVA, QUE PUEDE SER ESCINDIDA DE LOS ELEMENTOS DE PEPTIDO DESEADO (Pep) Y LOS ELEMENTOS DE PEPTIDO AUXILIAR (Aux) DE LA FORMULA GENENRAL (Pep-Aux)x, (Aux-Pep)x, DONDE x>1 LOS ELEMENTOS Aux SON IDENTICOS O DIFERENTES Y COMPRENDEN ELEMENTOS DE SECUENCIAS DE AMINOACIDOS QUE IMPARTEN A DICHA PROTEINA PRECURSORA PROPIEDADES DE AUTOENSAMBLAJE LOS ELEMENTOS Pep SON IDENTICOS O DIFERENTES Y COMPRENDEN LAS SECUENCIAS DE AMINOACIDOS DE MOLECULAS PEPTIDICAS IDENTICAS O DIFERENTES Y COMPRENDEN UNA SECUENCIA DE AMINOACIDOS SELECCIONADA DE SEQ ID NO: 20 o SEQ ID NO: 29 A 67. LOS ELEMENTOS Pep Y Aux FORMAN ENLACES PEPTIDICOS ENTRE SI Y EL ENLACE PEPTIDICO ES ESPECIFICAMENTE ESCINDIBLE QUIMICAMENTE O ENZIMATICAMENTE ADEMAS, AL MENOS UN PEPTIDO Aux, COMPRENDE UN ELEMENTO DE PEPTIDO PROTECTOR (SU) Q
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