Provided is a seedling cultivation plug tray, comprising: a seedling cultivation plug tray (102), with at least one hole groove (110) formed on the seedling cultivation plug tray body; and at least one groove (104) arranged at a top end of the hole groove and able to be fitted with a plantation cup arranged in the hole groove, wherein the plantation cup is used for accommodating seedling cultivation seeds. A seedling cultivation system with the seedling cultivation plug tray comprises the seedling cultivation plug tray and the plantation cup, wherein the plantation cup is arranged in the hole groove of the seedling cultivation plug tray, and the groove at the top end of the hole groove can bear the plantation cup. After the seedling cultivation seeds grow into plant seedlings, the seedling cultivation system can directly transplant, when the plant seedlings are transplanted, the plant seedlings together with the plantation cup onto a plantation plate, so that the whole cultivation process is more convenient a
