The present invention relates to chimeric virus strains, particularly to the stable cold-adapted temperature sensitive Enterovirus 71 strains EV71:eTLLβP20 and EV71:TLLeC5 and to the stable chimeric enterovirus strain TLLeCA16. The present invention also relates to vectors containing the nucleotide sequences of these strains and vaccines containing these strains.本發明係關於嵌合病毒菌株,尤其關於穩定之適於冷之溫度敏感型腸病毒71菌株EV71:eTLLβP20及EV71:TLLeC5及穩定之嵌合腸病毒菌株TLLeCA16。本發明亦關於包含此等菌株之核苷酸序列之載體及包含此等菌株之疫苗。