UNIVERSITE CLAUDE BERNARD LYON 1;INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE;CENTRE LEON BERARD;NEOLYS DIAGNOSTICS;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
发明人:
NICOLAS FORAY,LARRY BODGI,SANDRINE PEREIRA
申请号：
FR1559961
公开号：
FR3040178A1
申请日:
2015.10.20
申请国别(地区):
FR
年份:
2017
代理人:
摘要：
A method of evaluating the response of a tumor to a DNA-breaking treatment from a sample of cells of said tumor wherein: (a) a cell sample is prepared from cells taken from said tumor； (b) subjecting said cell sample to DNA-disrupting treatment characterized by a D dose, (c) determining on said cell sample the average number of nuclear foci obtained with a pH2AX marker at the observation times t (these numbers means being respectively called NpH2AX (t)), said observation times t being the time t = 0 min (called t0, state before administration of the dose D) and at least one observation time selected from t = t1, t2 , t3 and t4 after dosing D； (d) determining at least one parameter or score for characterizing the response of the sample to said DNA-breaking processing, using at least the average numbers NpH2AX (t), and wherein method • t4 is a fixed value which represents the time for which the rate of DNA breaks reaches its residual value, • t3 is a fixed value which represents the time after which approximately 25% of the double-strand breaks (DSBs) are repaired in control cells from radio-resistant patients, • t2 is a fixed value that represents the time after which approximately 50% of CBDs are repaired in control cells from radiation-resistant patients, • t1 is a fixed value that represents the time after which the number of recognized CBDs reaches its maximum in control cells from radiation-resistant patients. Procédé d'évaluation de la réponse d'une tumeur à un traitement cassant l'ADN à partir d'un prélèvement de cellules de ladite tumeur dans lequel : (a) on prépare un échantillon cellulaire à partir des cellules prélevées sur ladite tumeur ； (b) on soumet ledit échantillon cellulaire à un traitement cassant l'ADN caractérisé par une dose D, (c) on détermine sur ledit échantillon cellulaire le nombre moyen de foci nucléaires obtenus avec un marqueur pH2AX aux temps d'observation t (ces nombres moyens étant appelé respective
