Greenhouse (1) comprising a roof structure (4), at least one row of multiple supporting columns (2a-2f) supporting at least a part of the roof structure (4), and a cultivation gutter (50a-50g) extending within the greenhouse (1) in the direction of the row and supported by successive supporting columns (2a-2f) in the row, wherein the cultivation gutter (50a-50g) has a center line extending in the longitudinal direction of the cultivation gutter (50a-50g) and extending through the successive supporting columns (2a-2f).